EFFECTS OF DAFLON(R) 500 MG ON POSTISCHEMIC MACROMOLECULAR LEAK SYNDROME IN STRIATED SKIN MUSCLE OF THE HAMSTER

We have recently shown that the purified micronized flavonoid fraction (90% diosmin and 10% hesperidin) Daflon(R) 500 mg attenuates reperfus ion injury in the striated skin muscle of the hamster, Herein, we repo rt on the action of Daflon(R) 500 mg on postischemic macromolecular le akage of FITC-dextran 150 kD provoked by tourniquet ischemia, Intravit al fluorescence microscopy was used for analysis of macromolecular lea kage in the microcirculation model of the hamster. A tourniquet ischem ia of 4 h duration was induced followed by reperfusion. Animals were t reated by gavage of Daflon(R) 500 mg (n = 6) for 8 days at a daily dos e of 30 mg kg(-1) body weight, Control animals received equivalent vol umes of the vehicle (5% Arabic gum solution, n = 6), Measurements of t he microcirculatory parameters were made before induction of ischemia and at 0.5, 2 and 24 h of reperfusion, After induction of ischemia, ma cromolecular leakage from postcapillary venules was significantly enha nced in vehicle-treated animals, Treatment with Daflon(R) 500 mg signi ficantly attenuated macromolecular leakage of FITC-dextran 150 kD, Pre liminary data from a histomorphometric analysis (n = 3/experimental gr oup) indicated that the number of emigrated (extravascular) leukocytes after ischemia reperfusion was markedly reduced in Daflon(R) 500 mg-t reated animals as compared to controls, These data indicate that Daflo n(R) 500 mg prevents leakage of the macromolecular tracer FITC-dextran 150 kD from postcapillary venules after postischemic reperfusion, pre sumably through an inhibitory action on the emigration of activated le ukocytes.