IMMUNOPHENOTYPE OF MYELOID CELLS IN MYELODYSPLASTIC SYNDROMES AND ITSCLINICAL IMPLICATIONS

Objective To explore the immunophenotype of myeloid cells in myelodysp lastic syndyomes (MDS) and its clinical implications. Methods A panel of monoclonal antibody was used to detect CD13+, CD33+, CD15+ and CD14 + antigens on the membrane surfaces of myeloid cells in the bone marro w from 51 MDS, 21 aplastic anemia (AA) 21 paroxysmal nocturnal hemoglo binuria (PNH) patients, 10 acute myeloblastic leukemia (AML) patients and 15 normal subjects by immunoenzymatic assay. The morphology and ch romosome karyotype of bone marrow cells of MDS patients were also exam ined. Results CD14+, CD13+ and CD33+ cells in the bone marrow were mor e in MDS patients than in normal controls, AA patients and PNH patient s. CD15+ cells in the bone marrow were less in MDS patients than in no rmal controls. Conclusions The percentages of CD14+, CD13+ and CD33+ p ositive cells in the bone marrow of MDS patients were related to the p ercentage of myeloblasts, the chromosomal aberrations and the response to treatment. It indicated that there is immunophenotypic misexpressi on of myeloid cells in MDS patients. Immunophenotype analysis of myelo id cells might be useful for the diagnosis and treatment of MDS patien ts.