CHARACTERIZATION OF MARINE TEMPERATE PHAGE-HOST SYSTEMS ISOLATED FROMMAMALA BAY, OAHU, HAWAII

To understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and th e types of interactions that occur between marine viruses and their ho sts, We isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained from concentrated water samples fr om Mamala Bay, Oahu, Hawaii, Two of the rod-shaped bacterial hosts wer e identified as Sphingomonas paucimobilis and Flavobacterium sp. All o f the phage isolates were tailed phages and contained double-stranded DNA. Two of the phage isolates had morphologies typical of the family Siphoviridae, while the other two belonged to the families Myoviridae and Podoviridae. The head diameters of these viruses ranged from 47 to 70.7 nm, and the tail lengths ranged from 12 to 146 nm, The burst siz es ranged from 7.8 to 24O phage/bacterial cell, and the genome sizes, as determined by restriction digestion, ranged from 36 to 112 kb, The members of the Siphoviridae, T-phi HSIC, and T-phi D0, and the member of the Myoviridae, T-phi D1B, were found to form lysogenic association s with their bacterial hosts, which were isolated from the same water samples, Hybridization of phage T-phi HSIC probe with lysogenic host g enomic DNA was observed in dot blot hybridization experiments, indicat ing that prophage T-phi HSIC was integrated within the host genome, Th ese phage-host systems are available for use in studies of marine lyso geny and transduction.