DESIGN AND EVALUATION OF USEFUL BACTERIUM-SPECIFIC PCR PRIMERS THAT AMPLIFY GENES-CODING FOR BACTERIAL 16S RIBOSOMAL-RNA

Authors
MARCHESI JR SATO T WEIGHTMAN AJ MARTIN TA FRY JC HIOM SJ WADE WG
Citation
Jr. Marchesi et al., DESIGN AND EVALUATION OF USEFUL BACTERIUM-SPECIFIC PCR PRIMERS THAT AMPLIFY GENES-CODING FOR BACTERIAL 16S RIBOSOMAL-RNA, Applied and environmental microbiology, 64(2), 1998, pp. 795-799
Citations number
15
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
2
Year of publication
1998
Pages
795 - 799
Database
ISI
SICI code
0099-2240(1998)64:2<795:DAEOUB>2.0.ZU;2-Q
Abstract
We report the design and evaluation of PCR primers 63f and 1387r for a mplification of 16S rRNA genes from bacteria. Their specificity and ef ficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S r RNA gene amplification in ecological and systematic studies than PCR a mplimers that are currently more generally used.