THIN-LAYER CHROMATOGRAPHY OF ALDOPENTOSE AND ALDOHEXOSE DERIVATIVES

To study the relationship between chemical structure and retention beh avior in TLC on silica gel, the retention constants of variously subst ituted 1,2-O-isopropylidene derivatives of aldohexoses and 1,2-O-cyclo hexylidene derivatives of aldopentoses were measured as a function of the composition of eight binary mobile phases. The slopes and intercep ts of the linear relationships between the retention constant (R-M) an d the logarithm of the volume fraction of the polar mobile phase compo nent were calculated and are discussed in relation to solute and mobil e phase characteristics. The R-F values and relative retention (Delta R-M) of compounds depend largely on the retention behavior of their su bstituents.