IMPROVED ANALYTICAL PROCEDURE FOR THE MEASUREMENT OF CAPTOPRIL IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AND ITS APPLICATION TO PHARMACOKINETIC STUDIES
Me. Franklin et al., IMPROVED ANALYTICAL PROCEDURE FOR THE MEASUREMENT OF CAPTOPRIL IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AND ITS APPLICATION TO PHARMACOKINETIC STUDIES, Journal of chromatography B. Biomedical sciences and applications, 705(1), 1998, pp. 47-54
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
An enhanced, sensitive CC-MS assay is presented for the highly specifi
c angiotensin-converting enzyme (ACE) inhibitor, captopril. This metho
d improves previously published assays by using solid NEM as stabilize
r in the collection tubes, a rapid extraction technique with dichlorom
ethane and back-extraction into base, a commercially available interna
l standard (thiosalicylic acid) and a capillary GC column. Captopril a
nd the internal standard are measured as their bis-pentafluorobenzyl d
erivatives. The assay was linear from 10 to 5000 ng/ml with a mean rec
overy following solvent extraction at 50, 200 and 1000 ng/ml of 77%. A
t mean values of 45.9, 187 and 980 ng/ml inter assay precision and acc
uracy were 4.0, 2.9 and 3.5% and 8.2, 6.5 and 3.1%, respectively. Anal
ysis of captopril concentrations in plasma samples from 20 volunteers
following oral administration of 100 mg of captopril provided the foll
owing pharmacokinetic data (mean +/- S.D.): C-max,,,, 1470 +/- 467 ng/
ml; AUC(0-infinity), 1736 +/- 481 ng/ml.h; T-max, 0.73 h; k(e), 0.468
+/- 0.122 h(-1); elimination half life, 1.58 +/- 0.41 h. (C) 1998 Else
vier Science B.V.