DETERMINATION OF CEFOXITIN IN SERUM AND TISSUE

A simple, rapid and sensitive method for the clean-up and analysis of cefoxitin in serum and tissue is described. Serum (0.5 mi) and tissue (100 mg) samples after homogenization underwent high speed centrifugat ion. Chromatography was performed on a mu Bondapak C-18,, cartridge us ing a mobile phase of 0.005 M potassium dihydrogen phosphate-acetonitr ile-glacial acetic acid (77.5:22:0.5, v/v/v) with a flow-rate of 2.0 m l/min. Ultraviolet detection occurred at 235 nm. The procedure produce d a linear curve for the concentration range 100-5000 ng/ml. The assay produced accurate, repeatable and rapid results for both tissue and s erum samples without the need for chemcial extraction. (C) 1998 Elsevi er Science B.V.