SENSITIVE METHOD FOR THE DETECTION OF FUMONISIN B-1 IN HUMAN URINE

Fumonisins are a group of carcinogenic mycotoxins that occur worldwide . This paper reports a sensitive method for the detection and quantita tion of fumonisin B-1, in human urine. Amberlite XAD-2 non-ionic polym eric adsorbent resin is used prior to strong anion-exchange (SAX) cart ridge clean-up. As much as 100 mi of undiluted human urine can be load ed onto the column. Recoveries obtained using this method were 93.6, 9 5.1 and 94.4% when samples were spiked with 10, 50 and 500 ng/ml of fu monisin B-1, respectively. This method is highly reproducible (R.S.D.< 5%) and gives good sample clean-up, which is suitable for HPLC analysi s. (C) 1998 Elsevier Science B.V.