PCR-BASED GENOTYPING OF MNSS BLOOD-GROUP - SUBTYPING OF M-ALLELE TO M-G AND M-T

PCR-based genotyping of MNSs blood group system was investigated in co mbination with restriction fragment length polymorphism (RFLP), single -strand conformation polymorphism (SSCP) and allele-specific PCR ampli fication (ASPA) techniques. M and N alleles are based on three nucleot ide substitutions in exon 2 and one base change (G or T) in an intron of glycophorin A locus. The latter single base change was also found a mong M alleles analyzed in this study, so that M allele appeared to be subdivided into M-G and M-T. All three alleles, M-G, M-T and N were i dentified clearly by RFLP or SSCP analysis following a single amplific ation. S and s alleles are based on one nucleotide substitution in exo n 3 of glycophorin B gene. Genotyping of Ss blood group system was als o explored by PCR-SSCP or ASPA analysis, and problems in the methods w ere discussed.