A NOVEL C-TERMINAL BINDING-PROTEIN (CTBP2) IS CLOSELY-RELATED TO CTBP1, AN ADENOVIRUS E1A-BINDING PROTEIN, AND MAPS TO HUMAN-CHROMOSOME 21Q21.3

A region of the C-terminus of adenovirus type 2/5 E1A protein has been associated with negative modulation of tumorigenicity, as well as the extent of oncogenic transformation. In contrast with the N-terminus o f the E1A protein, which has been extensively characterized and shown to associate with a number of cellular proteins, the function of the C -terminus is poorly understood. To date, a single 48-kDa protein, CTBP 1, has been shown to associate with this region. Here we report the id entification and sequence of a novel gene in human (CTBP2) and mouse ( Ctbp2), both highly related to CTBP1 and thus also likely to bind to t he E1A protein. We found that CTBP2 is expressed in all tissues tested , with a higher level of expression in the heart, skeletal muscle, and pancreas. We mapped CTBP1 and CTBP2 to human chromosomes 4p16 and 21q 21.3, respectively. (C) 1998 Academic Press.