AN EXPRESSION MAP FROM HUMAN-CHROMOSOME 14Q24.3

We have constructed an expression map of chromosome 14q24.3 between ma rkers D14S42 and D14S63. cDNA selection with YACs from 14q24.3 was use d to generate expressed sequence tags (ESTs). The localization of ESTs was confirmed on a YAC contig. PCR products of ESTs were used as prob es to screen cDNA libraries leading to the isolation of transcripts fo r known and unknown genes. In total, the expression map contains 7 kno wn genes previously mapped to 14q24.3, 6 cDNA transcripts, and 15 anon ymous ESTs, The addition of 21 unique transcribed loci from an approxi mate to 5- to 7-Mb region of chromosome 14q24.3 will facilitate future efforts to identify human disease genes from this region. (C) 1998 Ac ademic Press.