STABILITY-INDICATING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHODS FOR 5-FLUOROURIDINE IN AQUEOUS-SOLUTION

Two methods of reversed-phase high performance liquid chromatography ( RP-HPLC) were used to detect and quantify the degradation of 5-fluorou ridine (GAS 316-46-1, 5-FUR) in aqueous solution. The H-1-NMR techniqu e was used for indentifying the degradation products. Several assays w ere performed from different aqueous solutions of 5-FUR. The best resu lts (optimal resolution, reproducibility and rapidity) were achieved b y using Resolve(R) C-18 as stationary phase and a mix (96:4) of 50 mmo l/l ammonium dihydrogen phosphate (adjusted to pH 3.5 with phosphoric acid) and acetonitrile as mobile phase. Despite the 5-FUR hydrolysis t o 5-fluorouracil (5-FU) and D-ribose under moderate conditions in unli kely due to high stability of the nucleoside, these were the identifie d degradation products in assays carried out.