CHARACTERISTICS OF RABBIT CLC-2 CURRENT EXPRESSED IN XENOPUS OOCYTES AND ITS CONTRIBUTION TO VOLUME REGULATION

In the Xenopus oocyte heterologous expression system, the electrophysi ological characteristics of rabbit ClC-2 current and its contribution to volume regulation were examined. Expressed currents on oocytes were recorded with a two-electrode voltage-clamp technique. Oocyte volume was assessed by taking pictures of oocytes with a magnification of x 4 0. Rabbit ClC-2 currents exhibited inward rectification and had a hali de anion permeability sequence of Cl- greater than or equal to Br- >> I- greater than or equal to F-. ClC-2 currents were inhibited by 5-nit ro-2-(3-phenylpropylamino)benzoic acid (NPPB), diphenylamine-2 -carbox ylic acid (DPC), and anthracene-9-carboxylic acid (9-AC), with a poten cy order of NPPB > DPC = 9-AC, but were resistant to stilbene disulfon ates. These characteristics are similar to those of rat ClC-2, suggest ing rabbit ClC-2 as a counterpart of rat ClC-2. During a 30-min perfus ion with hyposmolar solution, current amplitude at -160 mV and oocyte diameter were compared among three groups: oocytes injected with disti lled water, oocytes injected with ClC-2 cRNA, and oocytes injected wit h ClC-2 Delta NT cRNA (an open channel mutant with NH2-terminal trunca tion). Maximum inward current was largest in ClC-2 Delta NT-injected o ocytes (-5.9 +/- 0.4 mu A), followed by ClC-2-injected oocytes (-4.3 /- 0.6 mu A), and smallest in water-injected oocytes (-0.2 +/- 0.2 mu A), whereas the order of increase in oocyte diameter was as follows: w ater-injected oocytes (9.0 +/- 0.2%) > ClC-2-injected oocytes (5.3 +/- 0.5%) > ClC-2 Delta NT-injected oocytes (1.1 +/- 0.2%). The findings that oocyte swelling was smallest in oocytes with the largest expresse d currents suggest that ClC-2 currents expressed in Xenopus oocytes ap pear to act for volume regulation when exposed to a hyposmolar environ ment.