DEVELOPMENT OF FLEXSURE(R) HP - AN IMMUNOCHROMATOGRAPHIC METHOD TO DETECT ANTIBODIES AGAINST HELICOBACTER-PYLORI

We describe a solid-phase immunochromatographic serologic test, FlexSu re(R) HP, to detect IgG antibodies against Helicobacter pylori. H. pyl ori colonize the stomach and proximal duodenum, cause ulcer disease an d mucosa-associated lymphoid tissue lymphoma, and have a role in the d evelopment of other disorders, including gastric adenocarcinoma. FlexS ure HP consists of a test strip, conjugate pad, and absorbent pad, in a novel reverse-flow chromatography format. In these studies, FlexSure HP was demonstrated to be specific for IgG antibodies against H. pylo ri. The reactive cutoff of the test was consistent with [C-13]urea bre ath test and commercially available ELISAs. FlexSure HP had 94% sensit ivity, 88% specificity, and 91% accuracy relative to [C-13]urea breath test; and 95% sensitivity, 94% specificity, and 95% overall agreement relative to high-molecular-mass cell-associated protein enzyme immuno assay (HM-CAP EIA). FlexSure HP is a simple-to-perform, visually read test requiring no specialized training, equipment, or instrumentation, and yields rapid, accurate, qualitative results.