DETECTION OF IMMUNOGLOBULIN-G AND IMMUNOGLOBULIN-A ANTIBODIES TO RUBELLA-VIRUS IN URINE AND ANTIBODY-RESPONSES TO VACCINE-INDUCED INFECTION

Urine and serum samples from 89 healthy volunteers and three healthy i ndividuals who underwent rubella vaccination were tested for immunoglo bulin G (IgG), IgA, and IgM to rubella virus (RV) by enzyme-linked imm unosorbent assay methods, Subjects with positive (n = 68) or negative (n = 21) results for serum IgG were exactly the same as those with the corresponding results for urinary IgG, Both urinary and serum Ige lev els remained elevated from the 3rd or 4th week after vaccination until the end of the study, Both urinary IgA and serum IgM levels tended to increase rapidly between the 3rd and 5th week and then gradually decr ease until the end of the study, but the levels of both remained posit ive except for one sample each at the end (26th week), On the other ha nd, the ratio of anti-RV IgA titer to anti-RV IgG titer in urine (urin ary anti-RV IgA/IgG ratio) increased rapidly between the 3rd and ?th w eek after vaccination and then rapidly returned to the ratio levels of the subjects positive for serum IgG from among the healthy volunteers , In summary, detection of urinary anti-RV IgG should be useful for sc reening for previous RV infection, and measurement of urinary anti-RV IgA/IgG ratio might be useful for diagnosing recent infection.