A MONOCLONAL-ANTIBODY SPECIFIC TO SURFACE-ANTIGEN ON CANDIDA-KRUSEI

A monoclonal antibody (MAb; MAb 6B3) which reacts specifically with a cell wall antigen found in all strains or isolates of Candida krusei w as developed. MAb 6B3 was extensively tested by immunofluorescence ass ay for cross-reaction with many Candida, Cryptococcus, Saccharomyces, Trichosporon, and Rhodotorula species and was found to react only with the species C. krusei. The specific epitope is expressed on the surfa ce of fungal cells and appears to reside on a protein moiety. Taking i nto account the increasing importance of fluconazole-resistant strains in nosocomial fungal infections, the very high degree of specificity of this MAb for C. krusei could be useful for the routine detection of C. krusei in culture or in tissue samples.