LOCALIZATION BY FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) OF HUMAN MITOCHONDRIAL POLYMERASE-GAMMA (POLG) TO HUMAN-CHROMOSOME BAND 15Q24-]Q26, AND OF MOUSE MITOCHONDRIAL POLYMERASE-GAMMA (POLG) TO MOUSE-CHROMOSOME BAND 7E, WITH CONFIRMATION BY DIRECT-SEQUENCE ANALYSIS OF BACTERIAL ARTIFICIAL CHROMOSOMES (BACS)

Authors
ZULLO SJ BUTLER L ZAHORCHAK RJ MACVILLE M WILKES C MERRIL CR
Citation
Sj. Zullo et al., LOCALIZATION BY FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) OF HUMAN MITOCHONDRIAL POLYMERASE-GAMMA (POLG) TO HUMAN-CHROMOSOME BAND 15Q24-]Q26, AND OF MOUSE MITOCHONDRIAL POLYMERASE-GAMMA (POLG) TO MOUSE-CHROMOSOME BAND 7E, WITH CONFIRMATION BY DIRECT-SEQUENCE ANALYSIS OF BACTERIAL ARTIFICIAL CHROMOSOMES (BACS), Cytogenetics and cell genetics, 78(3-4), 1997, pp. 281-284
Citations number
13
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
78
Issue
3-4
Year of publication
1997
Pages
281 - 284
Database
ISI
SICI code
0301-0171(1997)78:3-4<281:LBFIH(>2.0.ZU;2-Y
Abstract
Cloned cDNAs for the human mitochondrial DNA polymerase gamma (POLG) w ere identified by homology with the yeast mitochondrial DNA polymerase catalytic subunit (MIP). Fluorescence in situ hybridization (FISH) of human and mouse bacterial artificial chromosomes (BACs), hybridized b y radioactively labeled POLG cDNAs, mapped to human chromosome band 15 q24-->q26, as well as to mouse chromosome band 7E. Direct sequencing o f the BAC DNA without subcloning confirmed the presence of both human POLG and mouse mitochondrial DNA polymerase gamma (Polg) in the respec tive BACs.