CHEMICAL AND ENZYMATIC-SYNTHESIS OF GLYCOCONJUGATES 3 - SYNTHESIS OF LACTOSAMINE BY THERMOPHILIC GALACTOSIDASE CATALYZED GALACTOSYLATION ONA MULTIGRAM SCALE

Authors
FANG JW XIE WH LI J WANG PG
Citation
Jw. Fang et al., CHEMICAL AND ENZYMATIC-SYNTHESIS OF GLYCOCONJUGATES 3 - SYNTHESIS OF LACTOSAMINE BY THERMOPHILIC GALACTOSIDASE CATALYZED GALACTOSYLATION ONA MULTIGRAM SCALE, Tetrahedron letters, 39(9), 1998, pp. 919-922
Citations number
20
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
39
Issue
9
Year of publication
1998
Pages
919 - 922
Database
ISI
SICI code
0040-4039(1998)39:9<919:CAEOG3>2.0.ZU;2-S
Abstract
A library of thermophilic glycosidases was screened for lactosamine pr eparation from a transgalactosylation reaction between lactose and glu cosamine hydrochloride. Two enzymes showed excellent regioselectivity toward glucosamine hydrochloride. The product could be synthesized on a multigram scale and was easily separated and purified by subsequentl y eluting through cation and anion exchange resin columns. (C) 1998 El sevier Science Ltd. All rights reserved.