MOLECULAR-CLONING OF BOVINE THYROTROPIN-RELEASING-HORMONE RECEPTOR GENE

Authors
TAKATA M SHIMADA Y IKEDA A SEKIKAWA K
Citation
M. Takata et al., MOLECULAR-CLONING OF BOVINE THYROTROPIN-RELEASING-HORMONE RECEPTOR GENE, Journal of veterinary medical science, 60(1), 1998, pp. 123-127
Citations number
16
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary medical science → ACNP
ISSN journal
09167250
Volume
60
Issue
1
Year of publication
1998
Pages
123 - 127
Database
ISI
SICI code
0916-7250(1998)60:1<123:MOBTRG>2.0.ZU;2-V
Abstract
The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using P CR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV ) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaque s were screened to obtain several clones each containing the N-terminu s or C-terminus domain. The bovine TRHR gene encoded 398 amino acids a nd has a long intron. The identity of the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. RT-PCR analys is indicated TRHR mRNA to be expressed in the pituitary and brain.