DETERMINATION OF GENETIC STABILITY OF MICROPROPAGATED PLANTS OF GINGER USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

Authors
ROUT GR DAS P GOEL S RAINA SN
Citation
Gr. Rout et al., DETERMINATION OF GENETIC STABILITY OF MICROPROPAGATED PLANTS OF GINGER USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS, Zhongyang yanjiuyuan. zhiwuxue huikan, 39(1), 1998, pp. 23-27
Citations number
31
Categorie Soggetti
Plant Sciences
Journal title
Zhongyang yanjiuyuan. zhiwuxue huikan → ACNP
ISSN journal
00068063
Volume
39
Issue
1
Year of publication
1998
Pages
23 - 27
Database
ISI
SICI code
0006-8063(1998)39:1<23:DOGSOM>2.0.ZU;2-3
Abstract
Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of micropropagated plants of Zingiber officinale s cv. V3S18. Fifteen arbitrary decamers were used to amplify DNA from in vivo and in vitro plant material to assess the genetic fidelity. Al l RAPD profiles from micropropagated plants were monomorphic and simil ar to those of field grown control plants. No variation was detected w ithin the micropropagated plants. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to ce rtify genetic stability throughout the systems of micropropagation is discussed.