ASD-GFP VECTORS FOR IN-VIVO EXPRESSION TECHNOLOGY IN PSEUDOMONAS-AERUGINOSA AND OTHER GRAM-NEGATIVE BACTERIA

We describe the construction of promoter probe vectors designed for id entification of bacterial genes induced in vitro and/or in vivo and fo r measurement of gene expression levels for in vivo expression technol ogy These plasmids use the Pseudomonas aeruginosa aspartate beta-semia ldehyde dehydrogenase (asd) gene as a selectable marker and beta-galac tosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein with e nhanced fluorescence properties (mut3GFP, pIVET-GFP, 5.48 kb) as repor ter gene systems. The proposed strategies can be adapted for use in mo st Gram-negative bacteria.