PREPARATION OF PCR PRODUCTS FOR DNA-SEQUENCING

Authors
LEONARD JT GRACE MB BUZARD GS MULLEN MJ BARBAGALLO CB
Citation
Jt. Leonard et al., PREPARATION OF PCR PRODUCTS FOR DNA-SEQUENCING, BioTechniques, 24(2), 1998, pp. 314-317
Citations number
5
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
24
Issue
2
Year of publication
1998
Pages
314 - 317
Database
ISI
SICI code
0736-6205(1998)24:2<314:POPPFD>2.0.ZU;2-Y
Abstract
We demonstrate that routine PCR product analytical agarose gels can al so serve as preparative gels for quick DNA template purification befor e sequencing. The band of interest is excised, placed into a Gel Nebul izer(TM) inside a Micropure(TM) separator and rapidly purified in a si ngle centrifugation step. Gel-purified PCR product, suitable for manua l and automated sequencing, is delivered within 10 min.