TRANSIENT BETA-GLUCURONIDASE ACTIVITY AFTER INFILTRATION OF ARABIDOPSIS-THALIANA BY AGROBACTERIUM-TUMEFACIENS

Authors
RAKOUSKY S KOCABEK T VINCENCIOVA R ONDREJ M
Citation
S. Rakousky et al., TRANSIENT BETA-GLUCURONIDASE ACTIVITY AFTER INFILTRATION OF ARABIDOPSIS-THALIANA BY AGROBACTERIUM-TUMEFACIENS, Biologia plantarum, 40(1), 1998, pp. 33-41
Citations number
22
Categorie Soggetti
Plant Sciences
Journal title
Biologia plantarum → ACNP
ISSN journal
00063134
Volume
40
Issue
1
Year of publication
1998
Pages
33 - 41
Database
ISI
SICI code
0006-3134(1998)40:1<33:TBAAIO>2.0.ZU;2-0
Abstract
Transient expression of the beta-glucuronidase (GUS) gene introduced i nto Arabidopsis thaliana intact plants by T-DNA after vacuum infiltrat ion of Agrobacterium tumefaciens was followed. The first incidence of GUS activity was found 2 - 3 d after treatment and a peak of activity one week after treatment in both A. thaliana races, Columbia and C24, GUS activity was sharply increased by cultivation of Arabidopsis plant s at elevated temperature (29 degrees C) compared to cultivation at 25 degrees C, The density of inocula also influenced the GUS activity.