MICROPROPAGATION OF KARWINSKIA-PARVIFOLIA AND THE TRANSFER OF PLANTS TO EX-VITRO CONDITIONS

Zygotic embryos of Karwinskia parvifolia, isolated from seeds obtained from different regions of Mexico, were cultured on Woody Plant Medium (WPM) supplemented with 0.06 mu M indole-3-acetic acid, 0.03 mu M gib berellic acid, and 2 mu M 6-benzylaminopurine, The growth of embryos a nd multiplication of shoots from stem segments were achieved. Rooting of excised shoots could be initiated on basal WPM medium with prolonge d subculture period to 2 months, or on WPM medium supplemented with 10 mu M 1-naphthaleneacetic acid, Multiplication capacity of shoots and rooting of K, parvifolia differed in dependence on the origin of expla nt material, The shoot multiplication was much lower than that of Karw inskia humboldtiana. The rooting depended on the origin of K. parvifol ia seeds, The regenerated plants were successfully transferred to glas shouse.