EFFECTS OF 3 AZOLE DERIVATIVES ON THE LIPIDS OF DIFFERENT STRAINS OF SPOROTHRIX-SCHENCKII

The comparative effects of ketoconazole, itraconazole, and fluconazole on the lipids of five Sporothrix schenckii strains were investigated. Quantitative analysis of lipids and sterols was completed, as well as qualitative analysis of sterols, by thin-layer chromatography and ult raviolet spectrophotometry. Growth of the S. schenckii isolates in the presence of azole derivative concentrations below the minimum inhibit ory concentration (MIG) resulted in significant alterations in the lip id and sterol contents as compared with the control values. Furthermor e, lanosterol was detected in these azole-treated cells. These results were in complete agreement with the proposed mechanism of action of a zoles, which act by inhibiting ergosterol biosynthesis with a conseque nt accumulation of lanosterol. Concerning the MIC values, fluconazole was found to be the least effective drug. On the other hand, as determ ined from a comparison of the effects of the three azoles on the stero l content of the strains studied, no significant differences in effica cy were found among the tested drugs.