Mj. Gilpin et al., MORPHOLOGICAL AND MOLECULAR ANALYSIS OF ANDROGENETIC, SELFED AND BACKCROSSED PLANTS PRODUCED FROM A HORDEUM-VULGARE X H-BULBOSUM HYBRID, Plant breeding, 116(6), 1997, pp. 505-510
Anther culture (AC) was carried out on a fertile triploid hybrid betwe
en Hordeum vulgare L. (cultivated barley) and H. bulbosum L. (bulbous
barley grass) to determine whether AC-derived regenerants differed fro
m progeny obtained through selfing and backcrossing. Chromosome counts
were carried out on all plants and DNA was extracted from them to pre
pare Southern blots for molecular analysis. To identify true recombina
nts, the blots were probed with rye repetitive sequence probes (pSc119
.1 and pSc119.2), which hybridize strongly and specifically to H. bulb
osum DNA. Twenty probes that detect single-or low-copy sequences were
hybridized with Southern blots containing restricted DNA extracted fro
m 25 AC-derived plants, 11 selfed and six back-crossed progeny that sh
owed hybridizations with pSc119. Although restriction fragment length
polymorphisms (RFLPs) were only observed using probes that map to four
of the possible 14 chromosome arms, an introgression associated with
chromosome 6HS was frequently observed among plants derived from AC, s
elfing and backcrossing. Plants from AC differed from selfed and backc
rossed progeny in their chromosome number; unique RFLP bands that were
occasionally observed may indicate chromosomal rearrangements.