SUPPRESSION OF ANAPHYLACTIC REACTION IN MURINE BY SIEGESBECKIA-PUBESCENS

The aqueous extract of Siegesbeckia pubescens (SPAE) inhibited compoun d 48/80-induced systemic anaphylaxis 100% with the dose of 1.0, 0.5 mg /g body weight (BW) at 1 h before or 5 min, 10 min after intraperitone al injection of compound 48/80. The passive cutaneous anaphylaxis (PCA ) reaction also inhibited to 78.5% by oral administration of SPAE (1.0 mg/g BW). When SPAE pretreated on mice at concentrations ranging from 0.0001 to 1.0 mg/g BW, the serum histamine levels were reduced in a d ose-dependent manner. Moreover, SPAE (100-800 mu g/ml) dose-dependentl y inhibited the histamine release from peritoneal mast cells (RPMC) by compound 48/80 (5 mu g/ml). Analysis by microscopic appearance observ ation revealed that SPAE (500 mu g/ml) stabilized the RPMC membrane. T herefore, these findings indicate that SPAE inhibits anaphylactic reac tions through stabilization of mast cell membrane.