CHLOROGENIC ACID, AN ANTIOXIDANT PRINCIPLE FROM THE AERIAL PARTS OF ARTEMISIA-IWAYOMOGI THAT ACTS ON 1,1-DIPHENYL-2-PICRYLHYDRAZYL RADICAL

The antioxidant activity of Artemisia iwayomogi was determined by meas uring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl ( DPPH) radical. The methanol extract of A. iwayomogi showed strong anti oxidant activity, and thus fractionated with several solvents, The ant ioxidant activity potential of the individual fraction was in the orde r of ethyl acetate > n-butanol > water > chloroform > n-hexane fractio n. The ethyl acetate and n-butanol soluble fractions exhibiting strong antioxidant activity were further purified by repeated silica gel and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid wa s isolated as one of the active principles from the n-butanol fraction , together with the inactive components, 1-octacosanol, scopoletin, sc opolin, apigenin 7,4'-di-O-methylether, luteolin 6,3'-di-Omethylether (jaceosidin), apigenin 7-methylether (genkwanin), 2,4-dihydroxy-6-meth oxyacetophenone 4-O-beta-D-glucopyranoside and quebrachitol. The antio xidant activity of chlorogenic acid was comparable to that of L-ascorb ic acid, which is a well known antioxidant.