TYPING OF THE SHORT TANDEM REPEAT D8S347 LOCUS WITH DIFFERENT FLUORESCENCE MARKERS

The short tandem repeat (STR) locus D8S347 was analyzed by capillary e lectrophoresis. Sequencing data and a population study of 203 individu als from a southwestern German population are presented. We detected 1 2 different alleles, 340-388 bp in length, and found 40 different geno types. The heterozygosity index was 85.7%. Futhermore, we investigated the consequences of different fluorescent dyes, namely 6-FAM, HEX, an d ROX, on the ABI-calculated fragment sizes of defined (i.e., sequence d) alleles (348-376 bp in length). 6-FAM-labeled fragments appear to b e smaller than the corresponding HEX- or POX-labeled fragments. On ave rage, 6-FAM-labeled fragments differ by 3.52 bp from the sequencing da ta, HEX-labeled ones by 2.04 bp, and ROX-labeled ones by 1.42 bp. Gene rally, small alleles differ less from the expected sequencing data tha n larger ones.