DETECTION OF GB VIRUS-C HEPATITIS-G VIRUS-RNA IN SERUM BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION

Authors
BHARDWAJ B QIAN KP DETMER J MIZOKAMI M KOLBERG JA URDEA MS SCHLAUDER G LINNEN JM KIM JP DAVIS GL LAU JYN
Citation
B. Bhardwaj et al., DETECTION OF GB VIRUS-C HEPATITIS-G VIRUS-RNA IN SERUM BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION, Journal of medical virology, 52(1), 1997, pp. 92-96
Citations number
12
Categorie Soggetti
Virology
Journal title
Journal of medical virology → ACNP
ISSN journal
01466615
Volume
52
Issue
1
Year of publication
1997
Pages
92 - 96
Database
ISI
SICI code
0146-6615(1997)52:1<92:DOGVHV>2.0.ZU;2-I
Abstract
Three PCR methods based on the GB virus-C/hepatitis G virus (GBV-C/HGV ) 5'UTR and NS3 genomic region were used for the detection of GBV-C/HG V RNA in serum of 62 patients with chronic hepatitis C virus (HCV) inf ection. Ten of 62 (16%) patients were found to have GBV-C/HGV RNA, whi ch was confirmed by sequence analysis of the 5'UTR PCR amplicon. All m ethods appear to be specific, but methods based on the 5'UTR appear to be more sensitive. (C) 1997 Wiley-Liss, Inc.