COMPARISON OF THE PROTEIN-PATTERNS OF ERYSIPELOTHRIX-RHUSIOPATHIAE STRAINS BY SDS-PAGE AND AUTORADIOGRAPHY

The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabe lled with L-[S-35] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAG E) and autoradiography. The strains were found to differ from each oth er in their protein patterns, thus, it was concluded that the method a pplied could provide useful data for the identification of strains. Th e described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains c ompared. The results show that there is no significant correlation bet ween the type of the strains and the percentage of identical molecular weight fractions.