Ap. Zavitsanos et T. Alebickolbah, ENANTIOSELECTIVE DETERMINATION OF TERAZOSIN IN HUMAN PLASMA BY NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY, Journal of chromatography, 794(1-2), 1998, pp. 45-56
Citations number
5
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A sensitive and selective analytical method for the enantioselective d
etermination of terazosin in human plasma has been developed. The chro
matography is based on the normal-phase chiral separation utilizing th
e analogue prazosin as the internal standard. The detection involves t
he direct introduction of the normal-phase eluent into an electrospray
source followed by mass-selective detection. No pre-column derivatiza
tion was required prior to analysis. The chiral stationary phase used
was Chiralpak AD 100 mmX2.1 mm I.D. (10 mu m particle size). The metho
d was utilized to determine the concentrations of terazosin enantiomer
s in human subjects following a 5 mg single oral dose. Results were co
mpared with those from an enantioselective HPLC-fluorescence method fr
om the same subject plasma samples. The LC-MS results confirm with con
fidence that the two terazosin enantiomers have different elimination
profiles. (C) 1998 Elsevier Science B.V.