CASEIN PHOSPHOPEPTIDES FROM CASEIN MICELLES BY SUCCESSIVE DIGESTION WITH PEPSIN AND TRYPSIN

When milk is ingested, casein micelles will be successively digested b y pepsin in the stomach and trypsin in the intestine. Therefore, we di gested casein micelles successively with pepsin at pH 4.0 and trypsin at pH 7.0, and recovered casein phosphopeptides (CPP) as CPP-calcium p hosphate (CP) complexes. The CPP-CP complexes contained 248 mg of calc ium/g peptides and 175 mg of inorganic phosphorus/g peptides, which we re higher than those of CPP-CP complexes driven from acid-precipitated casein and casein micelles by tryptic digestion only. It contained mo re (alpha(S1)-CN-5P(f59-79) than the other CPP preparations did.