ESCHERICHIA-COLI TRANSFORMANT EXPRESSING THE GLUCOSE-DEHYDROGENASE GENE FROM BACILLUS-MEGATERIUM AS A COFACTOR REGENERATOR IN A CHIRAL ALCOHOL PRODUCTION SYSTEM

Authors
KATAOKA M ROHANI LPS WADA M KITA K YANASE H URABE I SHIMIZU S
Citation
M. Kataoka et al., ESCHERICHIA-COLI TRANSFORMANT EXPRESSING THE GLUCOSE-DEHYDROGENASE GENE FROM BACILLUS-MEGATERIUM AS A COFACTOR REGENERATOR IN A CHIRAL ALCOHOL PRODUCTION SYSTEM, Bioscience, biotechnology, and biochemistry, 62(1), 1998, pp. 167-169
Citations number
18
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
1
Year of publication
1998
Pages
167 - 169
Database
ISI
SICI code
0916-8451(1998)62:1<167:ETETGG>2.0.ZU;2-8
Abstract
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenas e (GDH) gene from Bacillus megaterium IWG3 was examined for use as a c ofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-o xobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-ove rproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH reg enerator with NADP(+) and glucose, similarly to commercially available GDH.