SIMPLE METHOD FOR DETECTING GLYCOPROTEINS DOT-BLOTTED OR ELECTRO-BLOTTED ON TO A POLYVINYLIDENE DIFLUORIDE MEMBRANE

Authors
KABUTO S MURAMOTO K
Citation
S. Kabuto et K. Muramoto, SIMPLE METHOD FOR DETECTING GLYCOPROTEINS DOT-BLOTTED OR ELECTRO-BLOTTED ON TO A POLYVINYLIDENE DIFLUORIDE MEMBRANE, Bioscience, biotechnology, and biochemistry, 62(1), 1998, pp. 188-189
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
1
Year of publication
1998
Pages
188 - 189
Database
ISI
SICI code
0916-8451(1998)62:1<188:SMFDGD>2.0.ZU;2-R
Abstract
A simple method is described for detecting glycoproteins which had bee n dot-blotted or electro-blotted on to a polyvinylidene difluoride (PV DF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel ele ctrophoresis. This method is based on the periodate oxidation of glyco proteins on a PVDF membrane with subsequent staining by the chromophor ic hydrazide, 4-N,N-dimethylamino-4'-azobenzene sulfonyl hydrazide. Th e glycoproteins could be visualized as red-colored spots or bands in t he range of 0.16-0.31 mu g.