COMPARATIVE-STUDIES OF PROTEIN CRYSTALLIZATION BY VAPOR-DIFFUSION ANDMICROBATCH TECHNIQUES

Numerous reports have been published in the literature which describe the crystallization of macromolecules by a variety of crystallization methods, including the vapour-diffusion and microbatch techniques. Thi s topical review compares the results of examples of proteins which we re crystallized by both vapour-diffusion and microbatch methods. The i nherent features of the vapour-diffusion and microbatch methods are di scussed and some specific conditions where one method appears more fav ourable than the other are reported. Guidelines for the conversion of crystallization conditions from vapour diffusion to microbatch (and vi ce versa) are also presented.