CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A FAMILY-26 ENDO-BETA-1,4 MANNANASE (MANA) FROM PSEUDOMONAS-FLUORESCENS SUBSPECIES CELLULOSA

Authors
SCOTT M PICKERSGILL RW HAZLEWOOD GP BOLAM D GILBERT HJ HARRIS GW
Citation
M. Scott et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A FAMILY-26 ENDO-BETA-1,4 MANNANASE (MANA) FROM PSEUDOMONAS-FLUORESCENS SUBSPECIES CELLULOSA, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 129-131
Citations number
24
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
1
Pages
129 - 131
Database
ISI
SICI code
0907-4449(1998)54:<129:CAPDSO>2.0.ZU;2-C
Abstract
Crystals of an endo-beta-mannanase (1,4-beta-D-mannohydrolase, E.C. 3. 2.1.78) from Pseudomonas fluorescens sub species cellulosa have been g rown by the hanging-drop technique at 291 K over a period of one to tw o weeks to maximal dimensions of 0.17 x 0.17 x 0.25 mm. These crystals belong to the space group R32 (or R3) with cell dimensions of a = b = 155.4 and c = 250.8 Angstrom (hexagonal setting) and contain three (s ix) molecules in the asymmetric unit. The crystals diffract to at leas t 3.2 Angstrom using a laboratory source and are suitable for structur e determination.