DETERMINATION OF HBA(1C) IN THE PRESENCE OF HEMOGLOBIN-VARIANTS - COMPARISON OF 3 HPLC TECHNIQUES

Authors
CARTA M DALLOLIO G SOFFIATI G
Citation
M. Carta et al., DETERMINATION OF HBA(1C) IN THE PRESENCE OF HEMOGLOBIN-VARIANTS - COMPARISON OF 3 HPLC TECHNIQUES, European journal of clinical chemistry and clinical biochemistry, 35(12), 1997, pp. 923-925
Citations number
5
Journal title
European journal of clinical chemistry and clinical biochemistry → ACNP
ISSN journal
09394974
Volume
35
Issue
12
Year of publication
1997
Pages
923 - 925
Database
ISI
SICI code
0939-4974(1997)35:12<923:DOHITP>2.0.ZU;2-B
Abstract
The presence of Hb variants may cause analytical interference in HbA(1 c), values measured with the HPLC techniques currently used in Italian laboratories. In this study performance of a new HPLC system, (HA-814 0, Menarini) was compared with two other traditional HPLC systems (HA- 8121, Menarini and Diamat, Bio-Rad). The HA-8140 system detects the Kb variant possibly present in an independent peak. The integration area of such a peak is not computed in the calculation of the percentage v alue of HbA(1c). In this manner the underestimation of values obtained with traditional HPLC systems is avoided.