Sk. Majumder et al., ACID AND ALKALINE-PHOSPHATASES ACTIVITIES IN THE HARD BROWN HEAD PORTION (HBHP) OF SILK WORM (BOMBYX-MORI L.) LARVAE, Journal of advanced zoology, 18(2), 1997, pp. 113-115
A preliminary study on acid and alkaline phosphatases in Hard Brown He
ad Portion (hbhp) of fifth age larvae of silkworm, Bombyx mori against
, synthetic substrate para-nitro phenyl phosphate disodium salt (PNPP)
showed that acid phosphatase (EC 3.1.3.2) activity is larger than alk
aline phosphatase (EC3.1.3.1) in hbhp. Both acid and alkaline phosphat
ases activities had the highest peak in 5th day of the last instar lar
vae. The acid phosphatase activity in the hbhp displayed first order r
eaction until 37.5 degrees C for at least 30 minutes. Reaction of this
enzyme was studied over 20-50 degrees C due to its thermostability.