The phytotoxin produced by Burkholderia gladioli was purified and char
acterized. The ultraviolet and visible absorption spectrum of the phyt
otoxin exhibited two maxima at 260nm and 399 nm in 80% aqueous methano
l. The molecular weight of this substance was determined as 193 and th
e molecular formula was decided as C7H7N5O2 by a high resolution FAB m
ass spectrometry. From these results, this substance was identified as
toxoflavin.