THE PRESENCE OF RNA SPLICING SIGNALS IN THE CDNA CONSTRUCT OF THE E2 GENE OF CLASSICAL SWINE FEVER VIRUS AFFECTED ITS EXPRESSION

E2 is the major neutralizing antigen for classical swine fever virus ( CSFV) infection. Previously, we have cloned and sequenced the E2 cDNA of Taiwan strain p97 by the reverse transcription-polymerase chain rea ction (RT-PCR) method from CSFV-infected tissue. The presence of RNA s plicing donor and acceptor sites were found in the cDNA sequence. In t his study, transfection of E2 cDNA into mammalian cells resulted in th e production of a spliced RNA. Site-directed mutagenesis of the donor and acceptor sites prevented the RNA splicing event and generated a fu ll length transcript in COS7 cells. Although the spliced E2 transcript has not been reported in natural infection of CSFV, this study sugges ted that the potential splicing sites affected the E2 gene expression when the plasmid-based E2 gene was introduced into mammalian cells. (C ) 1997 Elsevier Science B.V.