REVERSE-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC ANALYSIS OF HARPAGOSIDE, SCORODIOSIDE AND VERBASCOSIDE FROM SCROPHULARIA-SCORODONIA - QUANTITATIVE-DETERMINATION OF HARPAGOSIDE
A. Diaz et al., REVERSE-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC ANALYSIS OF HARPAGOSIDE, SCORODIOSIDE AND VERBASCOSIDE FROM SCROPHULARIA-SCORODONIA - QUANTITATIVE-DETERMINATION OF HARPAGOSIDE, Planta medica, 64(1), 1998, pp. 94-95
A reversed-phase high performance liquid chromatographic method has be
en developed for the determination of the main compounds (harpagoside,
scorodioside, and verbascoside) from differents samples of Scrophular
ia scorodonia. The chromatographic method has been validated and appli
ed for quantitative determination of harpagoside. The results show the
highest levels of harpagoside in the leaf extract. The purity and ide
ntity of peaks were controlled by diode-array detection and comparison
with standards.