ORDERED CLONED DNA MAP OF THE GENOME OF VIBRIO-CHOLERAE 569B AND LOCALIZATION OF GENETIC-MARKERS

By using a low-resolution macrorestriction map as the foundation (R. M ajumder et al., J. Bacteriol. 176:1105-1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain 569B o f Vibrio cholerae has been constructed, A cosmid library the size of a bout 4,000 clones containing more than 120 Mb of V. cholerae genomic D NA (40-genome equivalent) was generated. By combining landmark analysi s and chromosome walking, the cosmid clones were assembled into 13 con tigs covering about 90% of the V. cholerae genome. A total of 92 cosmi d clones were assigned to the genome and to regions defined by NotI, S fiI, and CeuI macrorestriction maps, Twenty-seven cloned genes, 9 rrn operons, and 10 copies of a repetitive DNA sequence (IS1004) have been positioned on the ordered cloned DNA map.