CHARACTERIZATION OF CHLAMYDIA-TRACHOMATIS L2-INDUCED TYROSINE-PHOSPHORYLATED HELA-CELL PROTEINS BY 2-DIMENSIONAL GEL-ELECTROPHORESIS

Chlamydia trachomatis is an obligate intracellular bacteria, inducing its own uptake in nonprofessional phagocytes either by phagocytosis or pinocytosis. We have previously shown that C. trachomatis L2 induces tyrosine phosphorylation of eukaryotic proteins upon their entry by ph agocytosis. In this paper we characterize the tyrosine-phosphorylated proteins by two-dimensional gel electrophoresis. In immunoblotting wit h anti-phosphotyrosine antibodies of C. trachomatis L2-infected HeLa c ells, but not with uninfected cells, two rows of spots were observed w ith a molecular mass of 69 and 71 kDa and pi from 5.0 to 5.2. In addit ion, a single spot of 100 kDa and pi 6.2 was observed.