ADENOASSOCIATED VIRUS VECTOR CONTAINING THE HERPES-SIMPLEX VIRUS THYMIDINE KINASE GENE CAUSES COMPLETE REGRESSION OF INTRACEREBRALLY IMPLANTED HUMAN GLIOMAS IN MICE, IN CONJUNCTION WITH GANCICLOVIR ADMINISTRATION

Adeno-associated virus (AAV) has attracted considerable interest as a potential vector for gene therapy because of its wide host range, high transduction efficiency, and lack of cytopathogenicity. In this exper iment, we evaluated the efficacy of AAV vector containing the herpes s implex virus thymidine kinase (HSV-tk) gene on human gliomas transplan ted into the brain of nude mice. Complete regression of the tumors was observed after multiple AAV-tk injections followed by intraperitoneal ganciclovir (GCV) administration, and the survival of mice treated wi th AAV-tk vector and GCV administration was markedly prolonged, These results suggest that AAV-tk vectors may be useful for gene therapy aga inst malignant gliomas in humans.