EFFECT OF CIS-UNSATURATED FATTY-ACIDS ON METH-A ASCITIC TUMOR-CELLS IN-VITRO AND IN-VIVO

Earlier studies performed both by us and by others have demonstrated t hat some n-3 and n-6 fatty acids can inhibit the growth of tumour cell s in vitro. Though studies done with various types of oils rich in n-3 and n-6 fatty acids did show that the tumour incidence and growth can be modified, there were relatively few studies wherein the anti-tumou r effects of individual free fatty acids were studied. Here we present results which suggest that free fatty acids (oleic acid (OA), linolei c acid (LA), alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), a rachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) can inhibit the growth of methylcholanthrene-induced sarco ma cells (Meth-A cells) in vitro. The order of potency of various fatt y acids on the growth of Meth-A cells was DHA > ALA > EPA > AA > GLA > LA > OA and their ID50 values were 10, 20, 35, 45, 68, 73 and 110 mu g/ml/1 x 10(4) cells, respectively. These results indicate that the in hibitory action of different types of n-3, n-6 and n-9 fatty acids on Meth-A cells does not depend on their unsaturation. Vitamin E could pa rtially block the cytotoxicity of these fatty acids indicating a possi ble role for free radicals. GLA, AA and EPA augmented the generation o f superoxide anion and lipid peroxidation in Meth-A cells indicating a possible correlation between the ability of fatty acids to augment fr ee radicals and their tumoricidal action. In an in vivo study, it was observed that OA, LA, ALA, GLA and EPA can prolong the survival of Met h-A-bearing mice when given intraperitoneally. Of all the fatty acids tested, surprisingly, LA was found to be the most potent in enhancing the survival of the tumour-bearing animals at all the concentrations t ested. Thus, these studies suggest that free fatty acids can inhibit M eth-A tumour cell proliferation both in vitro and in vivo. (C) 1998 El sevier Science Ireland Ltd.