Xj. Tian et al., APPLICATION OF A RAPID THIN-SECTION METHOD FOR OBSERVATIONS ON DECOMPOSING LITTER IN MOR HUMUS FORM IN A SUB-ALPINE CONIFEROUS FOREST, Ecological research, 12(3), 1997, pp. 289-300
Morphological changes in the decomposing litter of Abies spp. and Betu
la spp. in a mor humus form were studied by a rapid thin section metho
d. According to the morphological characteristics, the epidermis, meso
phyll and vascular bundle of Abies needle litter were classified into
four types: (i) newly fallen; (ii) slightly decomposed; (iii) moderate
ly decomposed; and (iv) greatly decomposed. The distribution of these
tissue types along the profile of the forest floor was then investigat
ed. The morphological changes in other litter types, such as branches,
scales and Betula leaves during decomposition were observed directly
with microscope and electron microscope. Five vertical thin sections a
nd 80 horizontal thin sections were used for these observations and in
vestigations. The decomposition of Abies litter was slower than that o
f Betula litter. The relative decomposition rate of the tissues was in
the order of mesophyll > vascular bundle > epidermis in Abies needles
; mesophyll greater than or equal to epidermis > vascular bundles in B
etula leaves; and inner bark > xylem > outer bark in both Abies and Be
tula branches. The last remains of the litter were usually stomata, se
gments of seminiferous scale and outer bark of Abies. The decompositio
n of plant litter occurred mainly within the L and F layers of the soi
l (0-5 cm in depth). Abies needles and Betula leaves completely disapp
eared at. depths of 0-6 cm and 0-4 cm, respectively, Branches disappea
red within the top of 5 cm and 6-8 cm for Betula and for Abies, respec
tively. The scales of Abies were most slowly decomposed in the soil la
yers.