DIRECT LABELING OF ANTIBODIES IGG WITH RE-186 USING SODIUM GLUCOHEPTONATE

Citation
Zh. Zhu et al., DIRECT LABELING OF ANTIBODIES IGG WITH RE-186 USING SODIUM GLUCOHEPTONATE, Radiochimica Acta, 79(2), 1997, pp. 105-108
Citations number
14
Journal title
ISSN journal
00338230
Volume
79
Issue
2
Year of publication
1997
Pages
105 - 108
Database
ISI
SICI code
0033-8230(1997)79:2<105:DLOAIW>2.0.ZU;2-B
Abstract
In the direct labeling of antibodies with Re-186, 188, the lower redox potential of ReO4- than TcO4- requires the addition of excess SnCl2 a nd a medium-chelating agent for stabilizing the excess of SnCl2 in sol ution. Through extensive tests, sodium glucoheptonate (GH) was chosen as an excellent stabilizer for SnCl2 and also the reduced Re(V) from a variety of chelators, such as citrate, cyclodextrin, tartrate, inosit ol, glucose, glycine, etc. ReO4- solution was then quantitatively redu ced for 2 h with newly prepared SnCl2(GH) solution. Then, we directly incorporated the reduced Re to the antibodies IgG modified with 135 fo rd of NaHSO3 and 3500 fold of 2-ME, and more than 90% of specific bind ing was yielded in 100-150 min at room temperature. TLC analysis indic ated that less than 5% of activity was in the colloid form. Radiolabel ed antibodies IgG were stable to the challenging of 700 fold of DTPA, and also showed fine in vivo stability.