PHYSICAL AND GENETIC-MAP OF THE GENOME OF STAPHYLOCOCCUS-CARNOSUS TM300

A genome map of Staphylococcus carnosus TM300, an important microorgan ism in the food industry and long used as a starter culture, was const ructed by pulsed-field gel electrophoresis of DNA fragments obtained a fter digestion with NotI, SfiI and ApaI. The size of the chromosome wa s estimated to be 2590 kb. The fragments were assembled into a physica l map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clust ers were positioned on the physical map by Southern hybridization anal ysis. The map provides a basis for further analysis of the S. carnosus chromosome.