J. Kimura et al., PRODUCTION AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST OVARIAN ANTIGEN OF THE COMMON TREE SHREW (TUPAIA-GLIS), ANAT HISTOL, 27(1), 1998, pp. 57-60
To study the ovarian function of the common tree shrew (Tupaia glis),
monoclonal antibodies (MAb) against its ovarian antigen were produced.
Several positive hybridomas were cloned and MAb-T2C9 (IgG) was examin
ed for reactivity. Strong immunoreactivity of MAb-T2C9 was localized w
ithin the cytoplasm of luteal cells and in the theca interna cells of
Graafian follicles. MAb-T2C9 reacted also with testicular interstitial
cells and adrenocortical cells, except those of the zona glomerulosa.
Similar reactions were seen in the goat and sheep. Western blotting a
nalysis of the ovine corpus luteum after reaction with MAb-T2C9 reveal
ed a single positive band of approximate to 60 kDa. These findings sug
gest that MAb-T2C9 recognized protein molecules related to steroid syn
thesis.